NMR of proteins :
Through a collaboration with a major European NMR centre, we offer NMR services
which can yield essential data for protein or pharma research. We also use NMR
in house for checking protein structuration in delicate cases.
Protein labelling 13C,15N
- Definition of constructs
- Multiconstruct approach
- Stability tests using thermofluorimetry
- Systematic use of activity assays (incl. delivery of constructs for screening by customer)
Data acquisition and analysis
- Access to 600 to 900MHz spectrometers and NMR specialists
Applications
- Validation of structuration for new targets
- Titration experiments with Hits , leads or products bound to labelled targets
- Backbone assignment of new targets
- Dynamics analysis and dynamics perturbations by ligand binding
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