tetracycline inducible cell lines
All our cell line references are tetracycline inducible. This offers many advantages over classical CMV promoter based cell
line including lower toxicity induced by the receptor expression during the cell growth phase, a more stable expression over
the time and a higher level of expression for most of our products (over million receptors per cell). Inducible cell
lines are also well suited for 13C 15N labelling on a short period of time with a limited background labelling.
Our receptor constructs are carrying a double twin -strep-tag at the N-Terminus and a 10 histidine tag at the C-Terminus
thus permitting affinity purification on streptactin and/or nickel/cobalt column but also immobilisation of the receptors
on beads or coated surface. Two TEV cleavage sites inserted between the tags and the receptor for tags removal.
Moreover, customized constructs cell lines can be developed at very attractive prices (5 000 €) within 6 weeks for
any reference of our catalogue.
Cell line for receptors not available in our catalogue offer
can be developed for the same price (5 000 €) within 2
Scale-up : Our CHO and HEK cell lines are well suited for
scale-up expression in roller bottles allowing to collect
1 to 2 g of cell pellet/bottle (detailed protocols are delivered
with the cell lines).
Bulk orders can be places for cell pellet or membrane preparations
at competitive prices.
Consignment and prices:
Our stable cell lines are delivered either in frozen vials
(worldwide) or in culture flasks at room temperature (Europe
only). In both cases we also supply 100 ml of serum which
has been validated for our cell lines, we leave to our customers
to validate various serum lots from their usual suppliers.
The prices of our cell line are fixed according to the following
- 5 000 € for the cell lines produced upon order (delivery
time 6 to 8 weeks)
- 6 000 € for the cell lines immediately available but
not yet characterised by radio binding.
- 8 000 € for the cell lines characterised by radio binding
and expressing less than 1 M rec/cell
- 10 000 € for the cell lines characterised by radio
binding and expressing between 1 et 5 M rec/cell.
- 12 000 € for cell line characterised by radio binding
and expressing more than 5 M rec/cell
All our stable cell lines are characterised by radio ligand
binding as soon as a radio ligand is commercially available.
This policy has the following consequences:
- You will pay a non characterised cell line for a lower price
than the characterised ones.
- If the characterisation by radio ligand does not lead to
significant specific binding (more than 1000 specific DPM
for 10 µg of membrane preparation issued from the cell
line), the cell line will be reimbursed.
Phage display selection (antibody or peptide)