Xray structure determination :
X-ray structure resolution may last from a few weeks for the most favourable cases to years of work. A key factor of success is the behaviour of the target protein in solution. The expertise of Bio-Xtal allows us to provide quickly a clear evaluation of the chance of success of a structure resolution study
Our proposed proof of concept study includes:
- Protein domain definition and cloning
- Analytical expression/purification of 2 to 5 constructs
- Bio-physical characterisation
- Crystallisation pre-screen.
As for all of our fee-for-service proposed expertise, we are applying a "no result- no fee" policy to our X-ray crystallography offer. Do not hesitate to
for more detail on our structure resolution services.
Our Structural biology service Key points
Gene to constructs
- Definition of constructs
- Multiconstruct approach
- Systematic use of activity assays (incl. delivery of constructs for screening
by customer)
Crystallisation to structure resolution
- Screening in nanodrop format, optimisation in nano or micro drop format
- Co-crystallization or soaking of ligands
- Data collection In house and/or synchrotron
Applications
- New targets
- Hits , leads or products bound to targets
- Protein protein complexes
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